About service
We offer sophisticated proteomics services that are customized for researchers, drug companies, and academic institutions. We leverage state-of-the-art mass spectrometry and bioinformatics to produce accurate and useful research data.
Because they have access to state-of-the-art technologies as well as experienced staff, our clients are assured accurate data, which enables them to make informed decisions and advance their research.
Key Components of Our Study Design
Initial Consultation and Needs Assessment
This is the part where our experts gather your research objectives, sample bases and the biological information that you seek to obtain. In this particular consultation, we refine the parameters of the conducted study, analyze its potential obstacles, and define the best fitting NGS-based proteomics methodology for the project in question.
Proteomics Analysis: Integration Of All Protein Components
The next stage in the project involves extensive proteomic studies based on advanced NGS technologies. You will obtain deep coverage on more than 6,000 human proteins present in your samples allowing for comprehensive protein expression and abundance characterization. Such dataset provides an opportunity for the analysis of the changes in the protein content, enabling the identification of differentially expressed proteins, diagnostic/search markers, and essential biochemical pathways within the context of your study.
Optimisation of Experimental Design
Constructing informative, reproducible experiments is part of what our team will assist you with. This involves choosing the right sequencing method, deciding whether to do targeted or global proteomics, sample size and controls. There are also technical replicates, various labeling designs and randomization to reduce bias and strengthen the quality of your results.
Library Preparation and Sequencing Plan
We can provide specific library preparation protocols for your samples, which would facilitate the conversion of samples into sequencing libraries in an optimum manner. Our strategy comprises of optimizing respective fragmentation, adapter ligation, and PCR amplification steps to attain the depth and coverage desired. Further, we choose also the most suitable sequencing depth necessary for your research and budget.
Data Analysis and Bioinformatics Pipeline
We design a custom bioinformatics pipeline to process the complex type and large scale of data generated from NGS based proteomic studies. This may include quality assurance checks, data housekeeping, protein detection and quantification and pathways analysis. Biostatistics is used in the complete project in the form of innovative computational methods by all bioinformaticians.
Interpretation and Reporting
After formulating data analysis strategies we submit detailed reports featuring research results, statistics and their graphical representation. Our specialists will help you to evaluate the outcomes and possibly pinpoint key factors, biomarkers or proteins, and offer relevant follow-up or additional studies or experiments.
Post-Study Support
We extend our clinical research support even after your study is finished, such as help with data confirmation and publication as well as give ideas and expected tasks for new studies. We aim to make certain that you derive maximum benefit from the NGS centric proteomics data.